Mesenchymal stem cells are sensitive to experience-induced stresses, such as phototoxicity or bleaching, present in fluorescence microscopy, the current method of choice for stem cell imaging. These types of stress lead to a limitation of the imaging possibilities of cultured cells.

Implementing the use of the 3D Cell Explorer microscope would avoid these disruptions and improve this fundamental research as samples require no preparation, allowing rapid, non-invasive, and laboratory-independent live observation. expertise in mesenchymal stem cells. Additionally, the 3D Cell Explorer laser uses 100 times less energy than the lowest energy laser in current fluorescent imaging approaches, making long-term imaging (up to several weeks) possible.

In the example provided of a spectacular cell division taking place in a living sample of human mesenchymal stem cells cultured with a low serum cell growth medium7 and observed under the 3D Cell Explorer, the characterization of the different stages and structures of mitosis was possible. Additional details and static images of the obtained high-quality images are described here.

Find out more in our detailed blog article: https://nanolive.ch/mitosis-in-mesenchymal-stem-cells

Technology:
3D Cell Explorer microscope from Nanolive, Switzerland.
The 3D Cell Explorer is a high-speed, high-resolution, non-invasive live cell imaging microscope capable of examining biological systems in depth. This allows you to record stunning 3D images of whole cells in just seconds and with higher resolution than any conventional microscope on the market.

For more information, visit nanolive.ch.

Please take the opportunity to connect and share this video with your friends and family if you find it useful.