NGS libraries are created by cutting DNA into small pieces of a specific size. This cutting is done using high-frequency sound waves or enzymes. Then, DNA sequences called adapters are added to each end of a DNA fragment. These adapters contain the information needed for sequencing. They also include an index to identify the sample. Finally, all unbound adapters are removed and the library is complete. Depending on the application, there may be a PCR step to increase the quantity of the library. A successful library will be the right size. It will also be of a high enough concentration for sequencing.

Watch the full video “Next Generation Sequencing – A Step-by-Step Guide to DNA Sequencing” here: https://youtu.be/WKAUtJQ69n8

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