Design, modify, analyze and maximize your CRISPR-Cas9 editing efficiency

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Introduced to :
LabRoots First Annual – CRISPR Virtual Events 2018

Presented by:
Kevin Holden, PhD – Head of Synthetic Biology, Synthego

Speaker biography:
Kevin Holden, PhD, is head of synthetic biology at Synthego in Redwood City, California. He is part of a team charged with integrating synthetic biology workflows, such as CRISPR genome engineering, into new automation platforms for cell engineering. Additionally, he oversees collaborations with key thought leaders in the CRISPR research community and supports commercial activities by coordinating field application technical support for Synthego's portfolio of genome engineering solutions. Kevin frequently represents Synthego at academic and industry conferences, showcasing the company's latest research and development efforts. Kevin has over 12 years of experience in biotechnology, including collaborative research and development in synthetic biology and genome engineering. He received his doctorate in microbiology with an emphasis in biotechnology from the University of California, Davis. He is originally from the United Kingdom and immigrated to the United States as a youth.

Online seminar:
Design, modify, analyze and maximize your CRISPR-Cas9 editing efficiency

Webinar summary:
Learn how to optimize the efficiency of CRISPR-Cas9 editing in cell lines and primary cell types using engineered synthetic sgRNAs. Includes tips for achieving maximum knockout and activation efficiency, experimental examples on how to optimize iPSC editing, and a detailed explanation of CRISPR design and ICE analysis tools.

Learning Objectives:
1. Learn how to use free software tools to design CRISPR guide RNAs and analyze CRISPR editing.
2. Learn some key experimental parameters for optimizing CRISPR in cell lines and primary cells.