In our previous videos we briefly introduced the CRISPR Cas9 system (http://youtu.be/1aJxXWkE3Ek) and presented the different tools and methods available for their expression in living systems (http://youtu.be/INC_kdr7I34) . Here we will review the criteria for gRNA design and introduce you to online programs that simplify this procedure. We invite you to watch our previous videos before continuing with this one.

For more information on how to design gRNAs for CRISPR Cas9 systems, please visit:
Knowledge base: https://info.abmgood.com/crispr-cas9-sgrna-design

Chop Chop Harvard: https://chopchop.rc.fas.harvard.edu/

*Please note: there is an error at 1:12. Cas9 has an HNH and RuvC1-like nuclease domain (in the video it was incorrectly labeled /"NHN/").

Want more tips, tricks, guides and protocols on how to knock out a gene using CRISPR? Join our free 4-week #CRISPRCrash course: http://info.abmgood.com/crispr-crash-course

For more resources on CRISPR, visit:
https://info.abmgood.com/crispr-cas9

*Spanish subtitles are also available

Watch the other videos in this series on CRISPR:
Presentation: https://youtu.be/1aJxXWkE3Ek
Methods and tools: https://youtu.be/INC_kdr7I34
CRISPR playlist: https://youtu.be/1aJxXWkE3Ek?listPLTt9kKfqE_0Ei8_rQsrfm01-zQtABTn0Z

Check out our other video series:
Adeno-associated virus: https://youtu.be/hYHbfQe5h-Q?listPLTt9kKfqE_0HfXQMX9RPgbcmSWMqVtlBf
Next generation sequencing: https://youtu.be/jFCD8Q6qSTM?listPLTt9kKfqE_0Gem8hIcJEn7YcesuuKdt_n

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